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vsv g envelope protein  (Addgene inc)


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    Structured Review

    Addgene inc vsv g envelope protein
    Vsv G Envelope Protein, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 3097 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/envelope+protein+plasmid+pcmv+vsv+g/pCMV-VSV-G+(Plasmid+%238454)/pm41922576-124-21-25
    Average 96 stars, based on 3097 article reviews
    vsv g envelope protein - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Sequencing:

    Article Title: Endoplasmic reticulum stress and proteasome pathway involvement in human podocyte injury with a truncated COL4A3 mutation
    Article Snippet: Four mutant COL4A3 sequences in G619R, G801R, C1616Y, and c.4317delA, respectively, were obtained by scaffold bridge PCR amplification (PrimeSTAR HS DNA Polymerase, TAKARA, Japan).Using aClonExpress IIOne StepCloning Kit (Vazyme Biotech, China), these sequences were then inserted into the multiple cloning site downstream cytomegalovirus (CMV) promoter in a pCDH-CMVATG2S3F-IRES-Blast lentiviral plasmid (Institute Pasteur of Shanghai, China); the CMVpromoter would lead to high expression of the inserted target gene. .. After target gene sequence confirmation by Sanger DNA sequencing (GENEWIZ, China), the constructed plasmids were then transfected into 293T cells combined with envelope protein plasmid pCMV-VSV-G (Addgene Plasmid #8454) and packaging plasmid pCMV-dR8.2 dvpr (Addgene Plasmid #8455) to produce a lentivirus with either overexpressed WT or mutant COL4A3 (G619R, G801R, C1616Y, and c.4317delA). ..

    Article Title: Endoplasmic reticulum stress and proteasome pathway involvement in human podocyte injury with a truncated COL4A3 mutation
    Article Snippet: Using a ClonExpress II One Step Cloning Kit (Vazyme Biotech, China), these sequences were then inserted into the multiple cloning site downstream cytomegalovirus (CMV) promoter in a pCDH-CMV-ATG2S3F-IRES-Blast lentiviral plasmid (Institute Pasteur of Shanghai, China); the CMV promoter would lead to high expression of the inserted target gene. .. After target gene sequence confirmation by Sanger DNA sequencing (GENEWIZ, China), the constructed plasmids were then transfected into 293T cells combined with envelope protein plasmid pCMV-VSV-G (Addgene Plasmid #8454) and packaging plasmid pCMV-dR8.2 dvpr (Addgene Plasmid #8455) to produce a lentivirus with either overexpressed WT or mutant COL4A3 (G619R, G801R, C1616Y, and c.4317delA). ..

    DNA Sequencing:

    Article Title: Endoplasmic reticulum stress and proteasome pathway involvement in human podocyte injury with a truncated COL4A3 mutation
    Article Snippet: Four mutant COL4A3 sequences in G619R, G801R, C1616Y, and c.4317delA, respectively, were obtained by scaffold bridge PCR amplification (PrimeSTAR HS DNA Polymerase, TAKARA, Japan).Using aClonExpress IIOne StepCloning Kit (Vazyme Biotech, China), these sequences were then inserted into the multiple cloning site downstream cytomegalovirus (CMV) promoter in a pCDH-CMVATG2S3F-IRES-Blast lentiviral plasmid (Institute Pasteur of Shanghai, China); the CMVpromoter would lead to high expression of the inserted target gene. .. After target gene sequence confirmation by Sanger DNA sequencing (GENEWIZ, China), the constructed plasmids were then transfected into 293T cells combined with envelope protein plasmid pCMV-VSV-G (Addgene Plasmid #8454) and packaging plasmid pCMV-dR8.2 dvpr (Addgene Plasmid #8455) to produce a lentivirus with either overexpressed WT or mutant COL4A3 (G619R, G801R, C1616Y, and c.4317delA). ..

    Article Title: Endoplasmic reticulum stress and proteasome pathway involvement in human podocyte injury with a truncated COL4A3 mutation
    Article Snippet: Using a ClonExpress II One Step Cloning Kit (Vazyme Biotech, China), these sequences were then inserted into the multiple cloning site downstream cytomegalovirus (CMV) promoter in a pCDH-CMV-ATG2S3F-IRES-Blast lentiviral plasmid (Institute Pasteur of Shanghai, China); the CMV promoter would lead to high expression of the inserted target gene. .. After target gene sequence confirmation by Sanger DNA sequencing (GENEWIZ, China), the constructed plasmids were then transfected into 293T cells combined with envelope protein plasmid pCMV-VSV-G (Addgene Plasmid #8454) and packaging plasmid pCMV-dR8.2 dvpr (Addgene Plasmid #8455) to produce a lentivirus with either overexpressed WT or mutant COL4A3 (G619R, G801R, C1616Y, and c.4317delA). ..

    Construct:

    Article Title: Endoplasmic reticulum stress and proteasome pathway involvement in human podocyte injury with a truncated COL4A3 mutation
    Article Snippet: Four mutant COL4A3 sequences in G619R, G801R, C1616Y, and c.4317delA, respectively, were obtained by scaffold bridge PCR amplification (PrimeSTAR HS DNA Polymerase, TAKARA, Japan).Using aClonExpress IIOne StepCloning Kit (Vazyme Biotech, China), these sequences were then inserted into the multiple cloning site downstream cytomegalovirus (CMV) promoter in a pCDH-CMVATG2S3F-IRES-Blast lentiviral plasmid (Institute Pasteur of Shanghai, China); the CMVpromoter would lead to high expression of the inserted target gene. .. After target gene sequence confirmation by Sanger DNA sequencing (GENEWIZ, China), the constructed plasmids were then transfected into 293T cells combined with envelope protein plasmid pCMV-VSV-G (Addgene Plasmid #8454) and packaging plasmid pCMV-dR8.2 dvpr (Addgene Plasmid #8455) to produce a lentivirus with either overexpressed WT or mutant COL4A3 (G619R, G801R, C1616Y, and c.4317delA). ..

    Article Title: Endoplasmic reticulum stress and proteasome pathway involvement in human podocyte injury with a truncated COL4A3 mutation
    Article Snippet: Using a ClonExpress II One Step Cloning Kit (Vazyme Biotech, China), these sequences were then inserted into the multiple cloning site downstream cytomegalovirus (CMV) promoter in a pCDH-CMV-ATG2S3F-IRES-Blast lentiviral plasmid (Institute Pasteur of Shanghai, China); the CMV promoter would lead to high expression of the inserted target gene. .. After target gene sequence confirmation by Sanger DNA sequencing (GENEWIZ, China), the constructed plasmids were then transfected into 293T cells combined with envelope protein plasmid pCMV-VSV-G (Addgene Plasmid #8454) and packaging plasmid pCMV-dR8.2 dvpr (Addgene Plasmid #8455) to produce a lentivirus with either overexpressed WT or mutant COL4A3 (G619R, G801R, C1616Y, and c.4317delA). ..

    Transfection:

    Article Title: Endoplasmic reticulum stress and proteasome pathway involvement in human podocyte injury with a truncated COL4A3 mutation
    Article Snippet: Four mutant COL4A3 sequences in G619R, G801R, C1616Y, and c.4317delA, respectively, were obtained by scaffold bridge PCR amplification (PrimeSTAR HS DNA Polymerase, TAKARA, Japan).Using aClonExpress IIOne StepCloning Kit (Vazyme Biotech, China), these sequences were then inserted into the multiple cloning site downstream cytomegalovirus (CMV) promoter in a pCDH-CMVATG2S3F-IRES-Blast lentiviral plasmid (Institute Pasteur of Shanghai, China); the CMVpromoter would lead to high expression of the inserted target gene. .. After target gene sequence confirmation by Sanger DNA sequencing (GENEWIZ, China), the constructed plasmids were then transfected into 293T cells combined with envelope protein plasmid pCMV-VSV-G (Addgene Plasmid #8454) and packaging plasmid pCMV-dR8.2 dvpr (Addgene Plasmid #8455) to produce a lentivirus with either overexpressed WT or mutant COL4A3 (G619R, G801R, C1616Y, and c.4317delA). ..

    Article Title: Endoplasmic reticulum stress and proteasome pathway involvement in human podocyte injury with a truncated COL4A3 mutation
    Article Snippet: Using a ClonExpress II One Step Cloning Kit (Vazyme Biotech, China), these sequences were then inserted into the multiple cloning site downstream cytomegalovirus (CMV) promoter in a pCDH-CMV-ATG2S3F-IRES-Blast lentiviral plasmid (Institute Pasteur of Shanghai, China); the CMV promoter would lead to high expression of the inserted target gene. .. After target gene sequence confirmation by Sanger DNA sequencing (GENEWIZ, China), the constructed plasmids were then transfected into 293T cells combined with envelope protein plasmid pCMV-VSV-G (Addgene Plasmid #8454) and packaging plasmid pCMV-dR8.2 dvpr (Addgene Plasmid #8455) to produce a lentivirus with either overexpressed WT or mutant COL4A3 (G619R, G801R, C1616Y, and c.4317delA). ..

    Plasmid Preparation:

    Article Title: Endoplasmic reticulum stress and proteasome pathway involvement in human podocyte injury with a truncated COL4A3 mutation
    Article Snippet: Four mutant COL4A3 sequences in G619R, G801R, C1616Y, and c.4317delA, respectively, were obtained by scaffold bridge PCR amplification (PrimeSTAR HS DNA Polymerase, TAKARA, Japan).Using aClonExpress IIOne StepCloning Kit (Vazyme Biotech, China), these sequences were then inserted into the multiple cloning site downstream cytomegalovirus (CMV) promoter in a pCDH-CMVATG2S3F-IRES-Blast lentiviral plasmid (Institute Pasteur of Shanghai, China); the CMVpromoter would lead to high expression of the inserted target gene. .. After target gene sequence confirmation by Sanger DNA sequencing (GENEWIZ, China), the constructed plasmids were then transfected into 293T cells combined with envelope protein plasmid pCMV-VSV-G (Addgene Plasmid #8454) and packaging plasmid pCMV-dR8.2 dvpr (Addgene Plasmid #8455) to produce a lentivirus with either overexpressed WT or mutant COL4A3 (G619R, G801R, C1616Y, and c.4317delA). ..

    Article Title: Bromhexine inhibits SARS-CoV-2 Omicron and variant pseudovirus infection via ACE2-targeted mechanisms
    Article Snippet: .. Lentiviral particles were generated by co-transfecting HEK-293T cells with 3 μg of the vector pLENTI_hACE2_PURO (Addgene plasmid #155295, Watertown, MA, United States), 2 μg of the packaging plasmid psPAX2 (Addgene #12259), and 1 μg of the envelope protein plasmid pCMV-VSV-G (Addgene #8454), using Lipofectamine 2000 (Invitrogen) following the manufacturer’s protocol. ..

    Article Title: Endoplasmic reticulum stress and proteasome pathway involvement in human podocyte injury with a truncated COL4A3 mutation
    Article Snippet: Using a ClonExpress II One Step Cloning Kit (Vazyme Biotech, China), these sequences were then inserted into the multiple cloning site downstream cytomegalovirus (CMV) promoter in a pCDH-CMV-ATG2S3F-IRES-Blast lentiviral plasmid (Institute Pasteur of Shanghai, China); the CMV promoter would lead to high expression of the inserted target gene. .. After target gene sequence confirmation by Sanger DNA sequencing (GENEWIZ, China), the constructed plasmids were then transfected into 293T cells combined with envelope protein plasmid pCMV-VSV-G (Addgene Plasmid #8454) and packaging plasmid pCMV-dR8.2 dvpr (Addgene Plasmid #8455) to produce a lentivirus with either overexpressed WT or mutant COL4A3 (G619R, G801R, C1616Y, and c.4317delA). ..

    Mutagenesis:

    Article Title: Endoplasmic reticulum stress and proteasome pathway involvement in human podocyte injury with a truncated COL4A3 mutation
    Article Snippet: Four mutant COL4A3 sequences in G619R, G801R, C1616Y, and c.4317delA, respectively, were obtained by scaffold bridge PCR amplification (PrimeSTAR HS DNA Polymerase, TAKARA, Japan).Using aClonExpress IIOne StepCloning Kit (Vazyme Biotech, China), these sequences were then inserted into the multiple cloning site downstream cytomegalovirus (CMV) promoter in a pCDH-CMVATG2S3F-IRES-Blast lentiviral plasmid (Institute Pasteur of Shanghai, China); the CMVpromoter would lead to high expression of the inserted target gene. .. After target gene sequence confirmation by Sanger DNA sequencing (GENEWIZ, China), the constructed plasmids were then transfected into 293T cells combined with envelope protein plasmid pCMV-VSV-G (Addgene Plasmid #8454) and packaging plasmid pCMV-dR8.2 dvpr (Addgene Plasmid #8455) to produce a lentivirus with either overexpressed WT or mutant COL4A3 (G619R, G801R, C1616Y, and c.4317delA). ..

    Article Title: Endoplasmic reticulum stress and proteasome pathway involvement in human podocyte injury with a truncated COL4A3 mutation
    Article Snippet: Using a ClonExpress II One Step Cloning Kit (Vazyme Biotech, China), these sequences were then inserted into the multiple cloning site downstream cytomegalovirus (CMV) promoter in a pCDH-CMV-ATG2S3F-IRES-Blast lentiviral plasmid (Institute Pasteur of Shanghai, China); the CMV promoter would lead to high expression of the inserted target gene. .. After target gene sequence confirmation by Sanger DNA sequencing (GENEWIZ, China), the constructed plasmids were then transfected into 293T cells combined with envelope protein plasmid pCMV-VSV-G (Addgene Plasmid #8454) and packaging plasmid pCMV-dR8.2 dvpr (Addgene Plasmid #8455) to produce a lentivirus with either overexpressed WT or mutant COL4A3 (G619R, G801R, C1616Y, and c.4317delA). ..

    Generated:

    Article Title: Bromhexine inhibits SARS-CoV-2 Omicron and variant pseudovirus infection via ACE2-targeted mechanisms
    Article Snippet: .. Lentiviral particles were generated by co-transfecting HEK-293T cells with 3 μg of the vector pLENTI_hACE2_PURO (Addgene plasmid #155295, Watertown, MA, United States), 2 μg of the packaging plasmid psPAX2 (Addgene #12259), and 1 μg of the envelope protein plasmid pCMV-VSV-G (Addgene #8454), using Lipofectamine 2000 (Invitrogen) following the manufacturer’s protocol. ..



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